SOLID-PHASE MICROEXTRACTION OF MONOCYCLIC AROMATIC-AMINES FROM BIOLOGICAL-FLUIDS

A solid-phase microextraction (SPME) protocol was developed for the qu antitative analysis of monocyclic aromatic amines from biological flui ds, me headspace SPME sampling technique was optimized for extraction and concentration of five target analytes (aniline, o-toluidine, 2-chl oroaniline, 2,6-dimethylaniline, 2,4,6-trimethylaniline) from urine, b lood, and milk. The temperature, pH, and ionic strength of the matrix sample were modified to allow maximum adsorption of the analytes onto the SPME fiber. This method is rapid yet sensitive and can be complete d in 15 min on a 5-mL sample. SPME/GC/MS analysis yielded good reprodu cibility (RSD >11%) for each analyte from urine, blood, and milk, Meth od detection limits for the various biological fluids were determined and ranged from 0.40 ppb for 2,4,6-trimethylaniline in urine to 7.7 pp b for aniline in blood. This SPME sampling protocol can be applied to the biomonitoring of monocyclic aromatic amines from occupational, env ironmental, and medical exposure.