CONSTRUCTION AND CHARACTERIZATION OF AN ISOGENIC SLT-II DELETION MUTANT OF ENTEROHEMORRHAGIC ESCHERICHIA-COLI

Authors
GUNZER F BOHN U FUCHS S MUHLDORFER I HACKER J TZIPORI S DONOHUEROLFE A
Citation
F. Gunzer et al., CONSTRUCTION AND CHARACTERIZATION OF AN ISOGENIC SLT-II DELETION MUTANT OF ENTEROHEMORRHAGIC ESCHERICHIA-COLI, Infection and immunity, 66(5), 1998, pp. 2337-2341
Citations number
41
Categorie Soggetti
Immunology,"Infectious Diseases
Journal title
Infection and immunity → ACNP
ISSN journal
00199567
Volume
66
Issue
5
Year of publication
1998
Pages
2337 - 2341
Database
ISI
SICI code
0019-9567(1998)66:5<2337:CACOAI>2.0.ZU;2-W
Abstract
Enterohemorrhagic Escherichia coil (EHEC) produces Shiga-like toxins ( SLT), potent protein synthesis inhibitors. To further dissect the role of SLT-II in the course of disease, we have constructed E. coli TUV86 -2, an isogenic SLT-II-negative mutant of EHEC strain 86-24. The slt-i i gene was inactivated by suicide vector mutagenesis. We also isolated derivatives of strain 86-24 that were cured of the phage carrying the toxin genes.