Purpose: To evaluate the enamel fluoride uptake from a fluoride releas
ing pit and fissure sealant (Teethmate F). Materials and Methods: 16 m
axillary first premolars of young patients (10-12 years old), which we
re extracted for orthodontic reasons, were used. The children lived in
non-fluoridated areas. Eight teeth were sealed with Concise White Sea
lant and eight teeth with Teethmate P. Additionally, one maxillary fir
st premolar was sealed in vivo with Teethmate F and extracted for orth
odontic reasons 1 year after sealing its pits and fissures. Electron p
robe microanalysis was used for the area analysis of the fluoride upta
ke. Results: Teeth sealed with Concise White Sealant revealed no fluor
ide uptake while all teeth sealed with Teethmate F revealed high amoun
ts of fluoride uptake to a depth ranging from 10-20 mu m from the surf
ace. The premolar extracted 1 year after sealing with Teethmate F show
ed a similar fluoride uptake distribution in the enamel surface layer
as the samples prepared in vitro with the same sealant. The residual f
luoride was also observed within the sealing material.