CLONING OF THE RIBOSOMAL-PROTEIN L41 GENE OF PHAFFIA-RHODOZYMA AND ITS USE AS A DRUG-RESISTANCE MARKER FOR TRANSFORMATION

Authors
KIM IG NAM SK SOHN JH RHEE SK AN GH LEE SH CHOI ES
Citation
Ig. Kim et al., CLONING OF THE RIBOSOMAL-PROTEIN L41 GENE OF PHAFFIA-RHODOZYMA AND ITS USE AS A DRUG-RESISTANCE MARKER FOR TRANSFORMATION, Applied and environmental microbiology, 64(5), 1998, pp. 1947-1949
Citations number
25
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
64
Issue
5
Year of publication
1998
Pages
1947 - 1949
Database
ISI
SICI code
0099-2240(1998)64:5<1947:COTRLG>2.0.ZU;2-B
Abstract
The ribosomal protein L41 gene of Phaffia rhodozyma was cloned and use d as a dominant selectable marker for cycloheximide resistance in the transformation of P. rhodozyma. Electrotransformation with a plasmid c ontaining a ribosomal DNA fragment as a targeting signal typically yie lded 800 to 1,200 transformants/mu g of DNA with an integrated copy nu mber of about seven per haploid genome.