SUMMARY OF WORKSHOP FINDINGS FOR ANTIBODIES REACTING WITH PORCINE T-CELLS AND ACTIVATION ANTIGENS - RESULTS FROM THE 2ND INTERNATIONAL SWINE CD WORKSHOP

After initial evaluation of the 176 new and 19 control monoclonal anti bodies (mAb) submitted to the Second International Swine CD Workshop, 57 were assigned to the T-cell/activation marker subgroup. These 57 mA b were further analyzed using flow cytometry on whole blood lymphocyte s, splenocytes, Peyer's patch lymphocytes, in vitro cell lines, bronch o-alveolar lavage cells, Con A and PHA blasts, fetal cell populations, and by 2-color flow cytometry against mAb to porcine CD2, CD4: and CD 8. Finally, the molecular weights of the target antigens were characte rized when possible. As a result of these analyses, 23 mAb were distri buted into 7 CD clusters. Newly confirmed mAb assignments included: tw o CD2; one CD4; two CD5; one wCD6; and one wCD25. Three new mAb were f ound that reacted with wCD8, one of which defined a new epitope, wCD8c . For the first time, mAb against porcine CD3 were identified, includi ng 6 mAb that reacted with three different epitopes. Several new mAb r eacted with antigens whose expression varied depending on the activati on state of the test cell. These will require further characterization in order to assign a CD number. (C) 1998 Elsevier Science B.V.