PORCINE MYELOMONOCYTIC MARKERS - SUMMARY OF THE 2ND INTERNATIONAL SWINE CD WORKSHOP

Forty five mAbs submitted to the Second International Swine CD worksho p were analyzed by six different laboratories for their possible react ivity with porcine myelomonocytic cells using flow cytometry and immun ohistochemistry. As a result of these analyses, a new swine workshop c luster, SWC9, composed of two mAbs that recognize an antigen selective ly expressed on mature macrophages, was defined. In addition, several mAbs were identified, allowing the differentiation of granulocytes fro m monocytes/macrophages, or monocytes from macrophages. Further work i s required to identify the antigen recognized by these mAbs. Neverthel ess, they should already prove useful for the identification of differ ent stages in the macrophage maturation/differentiation, and will cert ainly aid analyses on the complexity of the mononuclear phagocyte syst em in the pig. Finally, the cross-reactivity of three anti-human CD14 mAbs with porcine myelomonocytic cells was established in this worksho p. (C) 1998 Elsevier Science B.V.