REACTIVITY OF WORKSHOP MONOCLONAL-ANTIBODIES ON PARAFORMALDEHYDE-FIXED PORCINE BLOOD MONONUCLEAR-CELLS

Authors
SCHUBERTH HJ RABE HU LEIBOLD W
Citation
Hj. Schuberth et al., REACTIVITY OF WORKSHOP MONOCLONAL-ANTIBODIES ON PARAFORMALDEHYDE-FIXED PORCINE BLOOD MONONUCLEAR-CELLS, Veterinary immunology and immunopathology, 60(3-4), 1998, pp. 409-417
Citations number
15
Categorie Soggetti
Immunology,"Veterinary Sciences
Journal title
Veterinary immunology and immunopathology → ACNP
ISSN journal
01652427
Volume
60
Issue
3-4
Year of publication
1998
Pages
409 - 417
Database
ISI
SICI code
0165-2427(1998)60:3-4<409:ROWMOP>2.0.ZU;2-X
Abstract
One hundred sixty-four monoclonal antibodies (mAbs) of the second inte rnational swine CD workshop were tested for their reactivity with porc ine blued mononuclear cells before and after fixing the cells with var ying concentrations of paraformaldehyde (PFA)(1, 5 ard 10 g l(-1)). A total of 38 (out of 134) positive reacting mAbs were significantly aff ected in their binding behavior on fixed cells. Modulation was seen as reduction in binding (staining intensity and/or % positive cells, n = 18) or in elevated values (n = 20). Modified mAb binding occurred aft er fixing cells with 5 to 10 g l(-1) PFA. (C) 1998 Elsevier Science B. V.