EPSTEIN-BARR-VIRUS GROWTH-TRANSFORMED CELLS ARE CONVERTED TO MALIGNANCY FOLLOWING TRANSFECTION OF A 1.3-KB CATR1 ANTISENSE CONSTRUCT INDEPENDENT OF A CHANGE IN THE LEVEL OF C-MYC EXPRESSION FOLLOWED BY A 8-14-CHROMOSOMAL TRANSLOCATION
Dw. Li et al., EPSTEIN-BARR-VIRUS GROWTH-TRANSFORMED CELLS ARE CONVERTED TO MALIGNANCY FOLLOWING TRANSFECTION OF A 1.3-KB CATR1 ANTISENSE CONSTRUCT INDEPENDENT OF A CHANGE IN THE LEVEL OF C-MYC EXPRESSION FOLLOWED BY A 8-14-CHROMOSOMAL TRANSLOCATION, Proceedings of the National Academy of Sciences of the United Statesof America, 95(9), 1998, pp. 4894-4899
The AGLCL Epstein-Barr virus (EBV) growth-transformed cell line is inc
apable of inducing tumors in nude mice. When the cells were transfecte
d with a 1.3-kb CATR1 antisense cDNA construct, progressively growing
lymphomas could be induced in nude mice. Chromosome analysis of the pa
rental, transfected, and tumor cells revealed that a chromosomal trans
location t(8;14)(q24.1;q32) had occurred in the transfected cells and
was retained in cells derived from tumors. Moreover, enhanced c-myc ex
pression, usually associated with this translocation, was either uncha
nged or under-expressed. These data suggest that the malignant transfo
rmation of the EBV growth-transformed cells was independent of c-myc e
xpression and suggest that the CATR1 gene mag act synergistically with
the chromosomal translocation facilitating the conversion of AGLCL ce
lls from a growth-transformed state to a malignant phenotype.