EPSTEIN-BARR-VIRUS GROWTH-TRANSFORMED CELLS ARE CONVERTED TO MALIGNANCY FOLLOWING TRANSFECTION OF A 1.3-KB CATR1 ANTISENSE CONSTRUCT INDEPENDENT OF A CHANGE IN THE LEVEL OF C-MYC EXPRESSION FOLLOWED BY A 8-14-CHROMOSOMAL TRANSLOCATION

Citation
Dw. Li et al., EPSTEIN-BARR-VIRUS GROWTH-TRANSFORMED CELLS ARE CONVERTED TO MALIGNANCY FOLLOWING TRANSFECTION OF A 1.3-KB CATR1 ANTISENSE CONSTRUCT INDEPENDENT OF A CHANGE IN THE LEVEL OF C-MYC EXPRESSION FOLLOWED BY A 8-14-CHROMOSOMAL TRANSLOCATION, Proceedings of the National Academy of Sciences of the United Statesof America, 95(9), 1998, pp. 4894-4899
Citations number
38
Categorie Soggetti
Multidisciplinary Sciences
ISSN journal
00278424
Volume
95
Issue
9
Year of publication
1998
Pages
4894 - 4899
Database
ISI
SICI code
0027-8424(1998)95:9<4894:EGCACT>2.0.ZU;2-Y
Abstract
The AGLCL Epstein-Barr virus (EBV) growth-transformed cell line is inc apable of inducing tumors in nude mice. When the cells were transfecte d with a 1.3-kb CATR1 antisense cDNA construct, progressively growing lymphomas could be induced in nude mice. Chromosome analysis of the pa rental, transfected, and tumor cells revealed that a chromosomal trans location t(8;14)(q24.1;q32) had occurred in the transfected cells and was retained in cells derived from tumors. Moreover, enhanced c-myc ex pression, usually associated with this translocation, was either uncha nged or under-expressed. These data suggest that the malignant transfo rmation of the EBV growth-transformed cells was independent of c-myc e xpression and suggest that the CATR1 gene mag act synergistically with the chromosomal translocation facilitating the conversion of AGLCL ce lls from a growth-transformed state to a malignant phenotype.