Jd. Keasling et Sw. Bang, RECOMBINANT-DNA TECHNIQUES FOR BIOREMEDIATION AND ENVIRONMENTALLY-FRIENDLY SYNTHESIS, Current opinion in biotechnology, 9(2), 1998, pp. 135-140
Citations number
49
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
A number of new recombinant DNA techniques have been developed for gen
etically engineered microorganisms for biodegradation of environmental
contaminants or for the synthesis of small molecules. These technique
s include new expression vectors to carry the heterologous genes into
the host organism, new mechanisms to control gene expression, containm
ent mechanisms to control persistence of genetically-engineered microo
rganisms, application of site-directed and random mutagenesis to incre
ase the substrate range or activity of biodegradative enzymes, and met
hods to track genetically-engineered microorganisms.