QUANTIFICATION OF 1,5-ANHYDRO-D-GLUCITOL IN URINE BY AUTOMATED BORATECOMPLEX ANION-EXCHANGE CHROMATOGRAPHY WITH AN IMMOBILIZED ENZYME REACTOR

HPLC using a berate form of a strongly anion-exchange resin column and an immobilized enzyme reactor for colorimetric detection was used to quantify urinary 1,5-anhydro-D-glucitol. Urine samples were introduced into the system every 7 min without any pretreatment, and after separ ation of interfering substances in the column, 1,5-anhydro-D-glucitol was successively detected. Quantitative determination of urinary 1,5-a nhydro-D-glucitol was possible within the 1.2-300 mu mol/l range. The coefficient of variance was less than 3% and the correlation between r esults obtained with our system (gamma) and those obtained by gas chro matography-mass spectrometry (x) was y=0.983x-1.287 mu mol/l (n=42, r= 0.998).