M. Solfrizzo et al., RAPID METHOD TO DETERMINE SPHINGANINE SPHINGOSINE IN HUMAN AND ANIMALURINE AS A BIOMARKER FOR FUMONISIN EXPOSURE/, Journal of chromatography B. Biomedical sciences and applications, 692(1), 1997, pp. 87-93
Citations number
17
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The widespread occurrence of fumonisins in maize and maize-based foods
and feeds demands the development of rapid and reliable methods for t
he analysis of suitable biomarkers in biological fluids in order to as
sess human and animal exposure to these important mycotoxins. The incr
ease in the ratio of free sphinganine/sphingosine (SA/SO) in urine has
been recently proposed as a biomarker to evaluate exposure to fumonis
ins. The presently available method for the determination of SA and SO
in biological samples is labor intensive, time consuming and insuffic
iently accurate. A new method has been proposed for the determination
of SA and SO in human and animal urine which is more precise and accur
ate, and drastically reduces the number of steps during extraction and
clean-up. The method is essentially based on the use of silica minico
lumn clean-up of the chloroform extract from alkalinized urine. The fi
nal extract is derivatized with o-phthaldialdehyde reagent and SO and
SA are determined by reversed-phase HPLC with fluorimetric detector. U
rine samples spiked with SO, SA standards at concentrations ranging fr
om 1.5 to 15 ng/ml have given mean recoveries higher than 80% and prec
ision (coefficient of variation) lower than 10%. Detection limit for S
O and SA was 0.1 ng/ml.