HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ACROLEIN AS A MARKER FOR CYCLOPHOSPHAMIDE BIOACTIVATION IN HUMAN LIVER-MICROSOMES

Authors
BOHNENSTENGEL F EICHELBAUM M GOLBS E KROEMER HK
Citation
F. Bohnenstengel et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ACROLEIN AS A MARKER FOR CYCLOPHOSPHAMIDE BIOACTIVATION IN HUMAN LIVER-MICROSOMES, Journal of chromatography B. Biomedical sciences and applications, 692(1), 1997, pp. 163-168
Citations number
20
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
692
Issue
1
Year of publication
1997
Pages
163 - 168
Database
ISI
SICI code
0378-4347(1997)692:1<163:HLDOAA>2.0.ZU;2-L
Abstract
A high-performance liquid chromatographic method for the quantificatio n of acrolein following incubation of cyclophosphamide (CP) with human liver microsomes was developed. Based on the formation of the fluores cent derivative 7-hydroxyquinoline by condensation of acrolein with 3- aminophenol quantitation was performed without prior extraction or oth er sample cleanup procedures. The method showed sufficient sensitivity with a limit of detection of 5 ng/ml and a limit of quantification of 10 ng/ml. The suitability of the method is shown for enzyme kinetic s tudies.