Pd. Laible et al., PROTEIN MODIFICATIONS AFFECTING TRIPLET ENERGY-TRANSFER IN BACTERIAL PHOTOSYNTHETIC REACTION CENTERS, Biophysical journal, 74(5), 1998, pp. 2623-2637
The efficiency of triplet energy transfer from the special pair (P) to
the carotenoid (C) in photosynthetic reaction centers (RCs) from a la
rge family of mutant strains has been investigated. The mutants carry
substitutions at positions L181 and/or M208 near chlorophyll-based cof
actors on the inactive and active sides of the complex, respectively.
Light-modulated electron paramagnetic resonance at 10 K, where triplet
energy transfer is thermally prohibited, reveals that the mutations d
o not perturb the electronic distribution of P. At temperatures greate
r than or equal to 70 K, we observe reduced signals from the carotenoi
d in most of the RCs with L181 substitutions. In particular, triplet t
ransfer efficiency is reduced in all RCs in which a lysine at L181 don
ates a sixth ligand to the monomeric bacteriochlorophyll B-B. Replacem
ent of the native Tyr at M208 on the active side of the complex with s
everal polar residues increased transfer efficiency. The difference in
the efficiencies of transfer in the RCs demonstrates the ability of t
he protein environment to influence the electronic overlap of the chro
mophores and thus the thermal barrier for triplet energy transfer.