PRENATAL DETECTION OF CHROMOSOME ANEUPLOIDIES BY FLUORESCENCE IN-SITUHYBRIDIZATION - EXPERIENCE WITH 2000 UNCULTURED AMNIOTIC-FLUID SAMPLES IN A PROSPECTIVE PRECLINICAL TRIAL
T. Bryndorf et al., PRENATAL DETECTION OF CHROMOSOME ANEUPLOIDIES BY FLUORESCENCE IN-SITUHYBRIDIZATION - EXPERIENCE WITH 2000 UNCULTURED AMNIOTIC-FLUID SAMPLES IN A PROSPECTIVE PRECLINICAL TRIAL, Prenatal diagnosis, 17(4), 1997, pp. 333-341
Successful rapid prenatal detection of selected numerical chromosome a
bnormalities by using fluorescence in situ hybridization (FISH) on unc
ultured amniotic fluid samples has been described by Klinger el al. (1
992) and Ward et al. (1993, 1997). Using essentially the same FISH pro
tocol and identical probes specific for chromosomes 21, 18, 13, X, and
Y, we prospectively compared the results of FISH and conventional cyt
ogenetics on 2000 amniotic fluid cell samples. The I-day FISH assay yi
elded discrete differences in the signal profiles between cytogenetica
lly disomic, i.e., normal, and trisomic samples. Due to intermittent a
bsent Y-signals, the assay differentiated less well between samples wi
th cytogenetically normal and abnormal sex chromosome complements. The
assay efficiency, and thus the clinical utility, was affected by (1)
unsuccessful hybridizations (7 per cent of all hybridizations), (2) hy
bridizations with less than 50 scorable nuclei (19 per cent of all hyb
ridizations), and (3) visibly contaminated samples with possible mater
nal cell contamination (14 per cent of all samples). As a result, we w
ere not able to reproduce the results of Klinger rt al. (1992) and War
d er al. (1993, 1997). (C) 1997 by John Wiley & Sons, Ltd.