HPLC ANALYSIS OF SOME SYNTHETIC PROSTAGLANDIN COMPOUNDS OF THERAPEUTIC INTEREST

The separation of some synthetic structural analogs of natural prostag landins PGF(2a), PGE(2) and PGA(2) was performed. A Beekman HPLC syste m equipped with reverse phase columns (Ultrasphere ODS 5 mu m) and wit h diode array detection was used with different mobile phases (methano l:water, methanol:0.75% acetic acid aqueous solution and methanol:0.02 M phosphate buffer). The optimal experimental conditions in relation with the chemical structure of each prostaglandin compound were establ ished. The quantitative determination of active compounds in the prese nce of different stereoisomers was also studied. The results of these studies were extended to quantitative determination of active prostagl andin compounds in pharmaceutical preparations (injectable solutions a nd collyria).