THE BIOCHEMICAL BASIS OF AN ALL-OR-NONE CELL FATE SWITCH IN XENOPUS OOCYTES

Xenopus oocytes convert a continuously variable stimulus, the concentr ation of the maturation-inducing hormone progesterone, into an all-or- none biological response-oocyte maturation, Here evidence is presented that the all-or-none character of the response is generated by the mi togen-activated protein kinase (MAPK) cascade. Analysis of individual oocytes showed that the response of MAPK to progesterone or Mos was eq uivalent to that of a cooperative enzyme with a Hill coefficient of at least 35, more than 10 times the Hill coefficient for the binding of oxygen to hemoglobin. The response can be accounted for by the intrins ic ultrasensitivity of the oocyte's MAPK cascade and a positive feedba ck loop in which the cascade is embedded. These findings provide a bio chemical rationale for the all-or-none character of this cell fate swi tch.