AN ENGINEERED RIBONUCLEASE PREFERRING PHOSPHOROTHIOATE RNA

Citation
S. Loverix et al., AN ENGINEERED RIBONUCLEASE PREFERRING PHOSPHOROTHIOATE RNA, Nature structural biology, 5(5), 1998, pp. 365-368
Citations number
33
Categorie Soggetti
Biophysics,Biology,"Cell Biology
Journal title
ISSN journal
10728368
Volume
5
Issue
5
Year of publication
1998
Pages
365 - 368
Database
ISI
SICI code
1072-8368(1998)5:5<365:AERPPR>2.0.ZU;2-L
Abstract
We used mutants of RNase Tl and the R-P isomer of a thiosubstituted su bstrate to determine stereospecific thioeffects on catalysis. The anal ysis reveals subtle structural and functional changes in the intermole cular transition state interactions. Tyr 38 contributes to catalysis t hrough a hydrogen bond with the pro-R-P oxygen. Y38F RNase Tl prefers the R-P thiosubstituted analog over the natural phosphodiester substra te that is favored by the wild type enzyme.