The masseter muscle is functionally heterogeneous with a complex archi
tecture consisting of multiple tendons and a multipennate arrangement
of muscle fibers. In this study, the anatomical partitioning of the ra
bbit masseter is described on the basis of the tendons of origin and i
nsertion, general partition orientation relative to the zygomatic arch
, motor endplate descriptions, and primary nerve branches that innerva
te these partitions. This work refines previous descriptions of the ra
bbit masseter and describes 13 anatomical partitions, each with a uniq
ue tendinous attachment. In addition, 14 naturally occurring primary n
erve branches were identified and found to innervate different regions
of the muscle, After correlating the anatomical partitions and the as
sociated neural innervation pattern, it was determined that simple bra
nch order will not adequately define all the neuromuscular compartment
s in the rabbit masseter.