SIMULTANEOUS VARIATION OF TEMPERATURE AND GRADIENT STEEPNESS FOR REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY METHOD DEVELOPMENT -I - APPLICATION TO 14 DIFFERENT SAMPLES USING COMPUTER-SIMULATION

The optimized reversed-phase HPLC separation of 14 different samples i s reported, based on simultaneous changes in temperature and gradient steepness. Four experimental runs are required for each sample, follow ing which preferred conditions can be predicted using computer simulat ion software (DryLab). The overall accuracy and effectiveness of this method development approach is discussed, with particular attention to the use of resolution maps provided by the software. These maps are u seful for maximizing resolution for the total sample, for optimizing t he separation of a smaller number of selected sample compounds, and as an initial step in the separation of more demanding samples. (C) 1998 Elsevier Science B.V.