SIMULTANEOUS VARIATION OF TEMPERATURE AND GRADIENT STEEPNESS FOR REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY METHOD DEVELOPMENT -II - THE USE OF FURTHER CHANGES IN CONDITIONS

The preceding paper (Part I) suggests that simply optimizing temperatu re and gradient steepness will often provide an adequate reversed-phas e HPLC separation. In some cases, however, this procedure will prove u nsuccessful, and then further method-development experiments (involvin g change in other separation conditions) will be required. One strateg y is to change a variable other than temperature or gradient steepness , followed by re-optimization of the latter two variables. The present paper examines the application of this approach with the aid of compu ter simulation to several samples. (C) 1998 Elsevier Science B.V.