CAPILLARY ELECTROPHORETIC AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHICSTUDIES OF THE ENANTIOSELECTIVE SEPARATION OF ALPHA-ADRENORECEPTOR ANTAGONISTS

Capillary electrophoresis (CE) and chiral stationary phase (CSP) HPLC methods were investigated for the determination of enantiomeric purity of alpha(1)-adrenoreceptor antagonists related to WE 4101. In the CE study, the enantioseparation of the analytes was performed by studying the effect of different types of cyclodextrin in the buffer, namely h eptakis (2,6-di-O-methyl)-beta-cyclodextrin (DMCD), hydroxypropyl-beta -cyclodextrin (HPCD) and beta-cyclodextrin (beta-CD). HPCD was found t o be the most effective chiral selector in the enantioseparation of al l the compounds, with high resolution values. A HPLC method, using imm obilised serum protein columns, human serum albumin (HSA) and alpha(1) -acid glycoprotein (AGP), was also investigated. Two benzodioxane race mates were well resolved on a mixed type (50% HSA and 50% AGP) column, with enantioselective binding on AGP column. (C) 1998 Elsevier Scienc e B.V.