Mhm. Dasilva et Pc. Debergh, THE EFFECT OF LIGHT QUALITY ON THE MORPHOGENESIS OF IN-VITRO CULTURESOF AZORINA-VIDALII (WATS.) FEER, Plant cell, tissue and organ culture, 51(3), 1997, pp. 187-193
In the present study, the possibility to improve the multiplication ra
te and quality of in vitro cultured plants of Azorina vidalii (Wats.)
Feer was investigated. For these purposes the influence of high and lo
w ratios of blue/red light (2.3 and 0.9, respectively) and red/far red
light (1.1 and 0.6, respectively) on the development of shoot culture
s of Azorina vidalii (Wats.) Feer was evaluated. Maximum plant length
(87.9 +/- 5.3 mm) (p less than or equal to 0.05) and internode length
(6.6 +/- 0.5 mm) (p less than or equal to 0.05) were obtained for the
plants cultured under the low ratio of red/far red light. In contrast,
when blue light was supplemented, plant height was reduced, due to sh
orter internodes. Highest number of axillary shoots per plant (2.4 +/-
0.5) was obtained in the plants growing under a high level of red/far
red light, while the lowest number (0.7 +/- 0.2) was obtained in the
plants cultured under the low level of red/far red light. Leaf area wa
s increased in plants cultured under a low level of red/far red (1,522
.5 +/- 129.5 mm(2)) while blue/red light treatments inhibited leaf exp
ansion. For chlorophyll a and total carotenoid contents, statistical d
ifferences (p less than or equal to 0.05) were observed between the pl
ants exposed to the red/far red light treatments and the plants from t
he control.