RADIOSYNTHESIS AND QUALITY ASSURANCE OF 5-[I-124]IODO-2'-DEOXYURIDINEFOR FUNCTIONAL PET IMAGING OF CELL-PROLIFERATION

5-[I-124]Iodo-2'-deoxyuridine ([I-124]IUdR) was routinely produced by direct electrophilic labelling of 2'-deoxyuridine with I-124 Of high s pecific activity (12 Ci/mu mol) in an Iodogen-coated ReactiVial, follo wed by purification on a Sep-Pak C-18 cartridge. The radiochemical pur ity was determined by TLC on a Silicagel-60 plate and by reverse phase HPLC on a RP-18 column. Based upon 45 syntheses, the yield ranged fro m 45% to 65%. The radiochemical impurity of [I-124]IUdR was determined at 2.9% by TLC (mainly iodate) and 4.3% by HPLC. The chemical stabili ty of the solvated formulation allowed a time window of 2 days followi ng end of synthesis (EOS) for chemical application, based upon the req uired 95% radiochemical purity grade of [I-124]IUdR. Th, labelled comp ound was routinely used for the clinical determination of cell prolife ration in glioma patients by positron emission tomography. (C) 1998 El sevier Science Inc.