CELLULAR-DISTRIBUTION OF IN-111-LDTPA GALACTOSE BSA IN NORMAL AND ASIALOGLYCOPROTEIN RECEPTOR-DEFICIENT MOUSE-LIVER

In-111-LDTPA galactose BSA (bovine serum albumin) was used to evaluate the asialoglycoprotein receptor (ASGPR) system in both normal and ASG PR-deficient mice. The radioiabeled glycoprotein had complete liver up take in both normal and ASGPR-deficient mice. Metabolism and hepatic c ell type distribution studies were performed. The normal mouse excrete d greater than 60% of the hepatic activity, while the ASGPR-deficient mouse excreted less than 40% of the hepatic activity. In-111-LDTPA gal actose BSA was metabolized to In-111-LDTPA L-lysine in both mouse type s. Normal mice showed 70% of the radioactivity in the hepatocyte, wher eas the homozygous ASGPR deficient mouse had equal activity in the hep atocyte and the hepatic endothelial cell. (C) 1998 Elsevier Science In c.