INTERACTION OF THE G-PROTEIN G(11)ALPHA WITH RECEPTORS AND PHOSPHOINOSITIDASE-C - THE CONTRIBUTION OF G-PROTEIN PALMITOYLATION AND MEMBRANEASSOCIATION

Authors
WISE A PARENTI M MILLIGAN G
Citation
A. Wise et al., INTERACTION OF THE G-PROTEIN G(11)ALPHA WITH RECEPTORS AND PHOSPHOINOSITIDASE-C - THE CONTRIBUTION OF G-PROTEIN PALMITOYLATION AND MEMBRANEASSOCIATION, FEBS letters, 407(3), 1997, pp. 257-260
Citations number
15
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
407
Issue
3
Year of publication
1997
Pages
257 - 260
Database
ISI
SICI code
0014-5793(1997)407:3<257:IOTGGW>2.0.ZU;2-N
Abstract
Wild-type and palmitoylation defective mutants of the murine G protein G(11)alpha were transfected into HEK293 tells. Wild-type G(11)alpha w as membrane associated, Cys9Ser Cys10Ser G(11)alpha was present in the soluble fraction whilst both Cys9Ser G(11)alpha and Cys10Ser G(11)alp ha were distributed between the fractions. Expression of the rat TRH r eceptor resulted in agonist stimulation of inositol phosphate accumula tion. The degree of stimulation produced by TRH following co-transfect ion of the palmitoylation-resistant forms of G(11)alpha compared to th e wildtype protein correlated with the amount of membrane-associated G protein. (C) 1997 Federation of European Biochemical Societies.