IDENTIFICATION OF WHEAT (TRITICUM-AESTIVUM L.) ANEUPLOIDS BY PROTEIN MARKERS

The wheat monosomics 1A, 1B, 1D and monotelodisomics 1AL, 1BL, 1DL ind ividuals were identified by electrophoretic analyses of high molecular weight glutenin subunits (HMW-GS) and gliadin blocks combined with qu antitative densitometric evaluations of glutenin and gliadin profiles. Absence of a whole chromosome or chromosome arm was confirmed by comp arison of relative quantity of corresponding HMW-GS or gliadin block e xpressed in aneuploid and euploid grains. Decreased quantity of expres sed protein is detectable in electrophoretic gels and is connected wit h absence of the corresponding chromosome or chromosome arm, i.e., wit h absence of the locus (or loci) controlling production of HMW-GS or g liadin proteins. The HMW-GS are useful molecular markers for simple, f ast, and unambiguous identification of wheat monosomics 1A, 1B, 1D, or monotelodisomics 1AS, 1BS, 1DS. Quantitative analysis of gliadin prof iles can facilitate identification of absent shea chromosome arms in m onotelodisomics 1AL, 1BL, and 1DL. Gliadin analyses can be used for id entification of monosomics 1A of wheat genotypes possessing the null a llele at locus Glu-A1.