DISCRIMINATION BETWEEN DIFFERENT TYPES OF HUMAN ADENOASSOCIATED VIRUSES IN CLINICAL-SAMPLES BY PCR

Persistent infection of human tissues with the helper virus-dependent parvovirus: adeno-associated virus (AAV) was detected by polymerase ch ain reaction (PCR) using primer pairs detecting AAV types 2, 3 or 5. I n order to develop PCR protocols which discriminate between the differ ent serotypes of AAV, the DNA of AAV-5 was sequenced partially and com pared with the published sequences of AAV-2 and -3. Type specific olig onucleotides and specific probes which allow the distinction between h uman AAV types by PCR are described. (C) 1998 Elsevier Science B.V. Al l rights reserved.