E. Kinoshita et al., ACTIVATION OF MAP KINASE CASCADE INDUCED BY HUMAN PANCREATIC PHOSPHOLIPASE A(2) IN A HUMAN PANCREATIC-CANCER CELL-LINE, FEBS letters, 407(3), 1997, pp. 343-346
We have found that the growth of human pancreatic cancer cells MIAPaCa
-2, induced by human pancreatic phospholipase A(2) group I (hPLA(2)-I)
, is mediated via its specific receptor but not via its catalytic prop
erty. The present study showed that the activation of mitogen-activate
d protein kinase (MAPK) cascade in MIAPaCa-2 cells is induced by hPLA(
2)-I: this digestive enzyme induced phosphorylation of MEK1/2, p44/42
MAPK and ATF-2, and the phosphorylation in the MAPK cascade was inhibi
ted after the cells were pre-incubated with a selective inhibitor of M
EK, PD98059. In addition, this inhibitor dose-dependently blocked the
hPLA(2)-I-induced MIAPaCa-2 proliferation, suggesting that activation
of the MAPK cascade is essential for the hPLA(2)-I-induced MIAPaCa-2 p
roliferation. (C) 1997 Federation of European Biochemical Societies.