ACTIVATION OF MAP KINASE CASCADE INDUCED BY HUMAN PANCREATIC PHOSPHOLIPASE A(2) IN A HUMAN PANCREATIC-CANCER CELL-LINE

Citation
E. Kinoshita et al., ACTIVATION OF MAP KINASE CASCADE INDUCED BY HUMAN PANCREATIC PHOSPHOLIPASE A(2) IN A HUMAN PANCREATIC-CANCER CELL-LINE, FEBS letters, 407(3), 1997, pp. 343-346
Citations number
28
Categorie Soggetti
Biophysics,Biology
Journal title
ISSN journal
00145793
Volume
407
Issue
3
Year of publication
1997
Pages
343 - 346
Database
ISI
SICI code
0014-5793(1997)407:3<343:AOMKCI>2.0.ZU;2-4
Abstract
We have found that the growth of human pancreatic cancer cells MIAPaCa -2, induced by human pancreatic phospholipase A(2) group I (hPLA(2)-I) , is mediated via its specific receptor but not via its catalytic prop erty. The present study showed that the activation of mitogen-activate d protein kinase (MAPK) cascade in MIAPaCa-2 cells is induced by hPLA( 2)-I: this digestive enzyme induced phosphorylation of MEK1/2, p44/42 MAPK and ATF-2, and the phosphorylation in the MAPK cascade was inhibi ted after the cells were pre-incubated with a selective inhibitor of M EK, PD98059. In addition, this inhibitor dose-dependently blocked the hPLA(2)-I-induced MIAPaCa-2 proliferation, suggesting that activation of the MAPK cascade is essential for the hPLA(2)-I-induced MIAPaCa-2 p roliferation. (C) 1997 Federation of European Biochemical Societies.