ANALYTICAL EVALUATION OF AN IMPROVED PROCEDURE FOR MEASURING THYROTROPIN

Citation
B. Gonzalezdelapresa et al., ANALYTICAL EVALUATION OF AN IMPROVED PROCEDURE FOR MEASURING THYROTROPIN, CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 36(2), 1998, pp. 103-106
Citations number
9
Categorie Soggetti
Biology
ISSN journal
14346621
Volume
36
Issue
2
Year of publication
1998
Pages
103 - 106
Database
ISI
SICI code
1434-6621(1998)36:2<103:AEOAIP>2.0.ZU;2-L
Abstract
The analytical characteristics of the AxSYM Ultrasensitive hTSH-II (Ab bott Laboratories) procedure for quantitation of serum thyrotropin (TS H) concentration were evaluated. Within-and between-run imprecisions, functional sensitivity, analytical interval and relative inaccuracy wi th respect to the Enzymum-Test TSH (Boehringer Mannheim) were studied. In all cases, the within-run and between-run of coefficients variatio n were lower than 6.69% and 8.12% respectively. The measurement range was tested with serial dilutions of a serum with a high thyrotropin co ncentration, and the procedure was found to be linear up to at least 8 7.0 mIU/l. The functional sensitivity was 0.018 mIU/l. The relative in accuracy study (Passing-Bablok non-parametric linear regression) produ ced the following linear equation: (AxSYM) = 1.02. (ES-700) - 0.03 mIU /l, with 95% confidence intervals of a (-0.05; -0.01); b (0.98; 1.06).