DETECTION OF DERMONECROTIC TOXIN GENE IN PASTEURELLA-MULTOCIDA STRAINS USING POLYMERASE CHAIN-REACTION

Authors
HOTZEL H ERLER W SCHIMMEL D
Citation
H. Hotzel et al., DETECTION OF DERMONECROTIC TOXIN GENE IN PASTEURELLA-MULTOCIDA STRAINS USING POLYMERASE CHAIN-REACTION, Berliner und Munchener Tierarztliche Wochenschrift, 110(4), 1997, pp. 139-142
Citations number
12
Categorie Soggetti
Veterinary Sciences
Journal title
Berliner und Munchener Tierarztliche Wochenschrift → ACNP
ISSN journal
00059366
Volume
110
Issue
4
Year of publication
1997
Pages
139 - 142
Database
ISI
SICI code
0005-9366(1997)110:4<139:DODTGI>2.0.ZU;2-P
Abstract
A PCR method was developed which allows to distinguish between Pasteur ella multocida strains carrying or lacking the dermonecrotic toxin gen e. Specific primers were used to amplify a 1501-bp DNA fragment from t he genomic dermonecrotic toxin gene region. Isolated DNA, broth cultur es and swabs were used as samples. Detection of the toxin gene directl y from swab samples accelerates considerably the diagnosis since culti vation steps can be omitted. The results of PCR corresponded to findin gs obtained by ELISA.