A CYTOPLASMIC AAA FAMILY PEROXIN, PEX1P, INTERACTS WITH PEX6P

Human PEX1 (HsPEX1) is the causative gene for peroxisome-deficiency di sorders such as Zellweger syndrome of complementation group I, encodin g the peroxin, Pex1p, a member of AAA family. Pex1p tagged with an epi tope flag was expressed in wild-type Chinese hamster ovary (CHO) cell, CHO-K1. Pex1p was localized in the cytoplasm, as assessed by immunofl uorescent microscopy. Cell-lysate of HsPEX1-transfected CHO-K1 was inc ubated with in vitro synthesized S-35-labelled Pex6p, an AAA family pe roxin. Immunoprecipitation of Pex1p using anti-Pex1p antibody resulted in concomitant recovery of S-35-Pex6p. Conversely, S-35-Pex1p was obt ained in immunoprecipitate from CHO-K1 expressing human Pex6p, using a nti-Pex6p antibody. These results strongly suggest that Pex1p and Pex6 p interact with each other, (C) 1998 Academic Press.