ENANTIOMERIC SEPARATION OF AROMATIC AMINO ALCOHOL DRUGS BY CHIRAL ION-PAIR CHROMATOGRAPHY ON A SILICA-GEL PLATE

Four chiral aromatic amino alcohol drugs were separated by TLC on a ge neral-purpose silica gel plate with ammonium-D-10-camphorsulfonate (CS A) as chiral ion-interaction agent. The chiral aromatic amino alcohols are all of pharmacological importance as beta-adrenergic blockers. Th e influence of eluent composition, temperature and concentration of CS A on the chiral separation is discussed. It is found that the temperat ure is also one of the important parameters to be varied for optimum s eparation in ion-pair chiral resolution. Among four drugs, three enant iomeric drugs were resolved at lower temperature (5 degrees C). In thi s study, analytical reagent grade methanol and dichlormethane can be d irectly used as mobile phase without using molecular sieve before use.