Ms. Rahman et al., MONITORING OF ACUTE ALLOGRAFT-REJECTION BY CYTOLOGICAL, IMMUNOCYTOCHEMICAL, AND IMMUNOHISTOCHEMICAL STUDIES FOLLOWING RAT SMALL-BOWEL TRANSPLANTATION, SURGERY TODAY-THE JAPANESE JOURNAL OF SURGERY, 28(5), 1998, pp. 529-537
We investigated the role of graft luminal fluid cytology for immunolog
ical monitoring of rat small-bowel allograft recipients. Allogeneic tr
ansplantation from WKAM (RT1(u)) to Lewis recipients and syngeneic tra
nsplantation using Lewis (RT1(l)) rats were carried out. Twenty centim
eters of the proximal jejunum was transplanted as a Thiry-Vella loop.
The luminal fluid on days 0, 3, and 6 was examined cytologically using
Papanicolaou, periodic acid-Schiff, and Giemsa staining, and immunocy
tochemically with monoclonal antibodies for macrophages (ED1 and ED2),
Full thickness biopsies of graft tissue were evaluated by both immuno
fluorescence (ED1 and ED2) and by standard histological methods. The c
ytological examination on day 6 revealed an increase in the number of
enterocytes, lymphocytes, and neutrophils, the presence of bacteria, a
nd the depletion of goblet cells in the allografts, Histologically, si
gnificant morphological changes of acute rejection were first seen on
day 6, Immunofluorescence predicted the acute rejection of the allogra
fts earlier than a histological examination by shelving an increase in
the number of ED1- and Ed2-positive cells on day 3, Graft luminal flu
id cytology and immunofluorescence analysis of ED1 and ED2 cells could
thus be used to recognize early acute allograft rejection following s
mall-bowel transplantation.