P. Boer et al., CYCLIC-AMP DECREASES THE AVAILABILITY OF 5-PHOSPHORIBOSYL-1-PYROPHOSPHATE AND DECELERATES DE-NOVO PURINE SYNTHESIS IN RAT HEPATOCYTES, Life sciences, 62(23), 1998, pp. 2133-2139
Citations number
33
Categorie Soggetti
Biology,"Medicine, Research & Experimental","Pharmacology & Pharmacy
Cyclic adenosine monophosphate (cAMP) was found to decrease the availa
bility of 5-phosphoribosyl-1-pyrophosphate (PRPP) and to decelerate th
e rate of de novo purine synthesis in suspensions of adult rat hepatoc
ytes. Glucagon did not affect these parameters. The glucagon antagonis
t des-His(1)[Glu(9)]glucagon amide (DHGA), and the protein kinase C ac
tivator 1,2-dioctanoyl-sn-glycerol (DOG) were also found to lower PRPP
availability. Incubation of the hepatocytes with dbcAMP or with DHGA,
did not alter the activity of PRPP synthetase in the hepatocyte lysat
es, indicating that the above effects are not mediated through the act
ivity of this enzyme. The possibility that the decrease in PRPP availa
bility reflects increased consumption associated with accelerated pyri
midine synthesis is discussed. The decelerated rate of decreased PRPP
availability.