Ml. Haapalainen et al., INTEGRATIVE VECTOR FOR STABLE TRANSFORMATION AND EXPRESSION OF A BETA-1,3-GLUCANASE GENE IN CLAVIBACTER-XYLI SUBSP CYNODONTIS, FEMS microbiology letters, 162(1), 1998, pp. 1-7
Clavibacter xyli subsp. cynodontis is an endophytic Gram-positive bact
erium that is able to colonize the xylem tissue of various plants. We
constructed an integrative vector that can carry foreign genes into a
repetitive chromosomal element of C. xyli subsp. cynodontis. Using thi
s vector, we transformed C. xyli subsp. cynodontis with a gene coding
for an endoglucanase enzyme under the control of a strong promoter tha
t was previously isolated from the genome of C. xyli subsp. cynodontis
. The transformed bacteria efficiently expressed active glucanase and
secreted it into the culture medium. The vector has the advantage of b
eing stably maintained in the chromosome; the transformations were mai
ntained without antibiotic selection both in in vitro culture and in b
acteria growing in maize xylem. (C) 1998 Federation of European Microb
iological Societies. Published by Elsevier Science B.V.