THE BACILLUS-SUBTILIS GLPD LEADER AND ANTITERMINATOR PROTEIN GLPP PROVIDE A TARGET FOR GLUCOSE REPRESSION IN ESCHERICHIA-COLI

Expression of the Bacillus subtilis glpD gene which encodes glycerol-3 -phosphate (G3P) dehydrogenase is regulated by the GlpP protein which, in the presence of G3P, causes antitermination of transcription of gl pD. The glpD gene leader fused to lacZ was integrated into the chromos ome of Escherichia coli deleted for the lac operon and carrying the B, subtilis glpP gene on a plasmid. beta-Galactosidase activity of this strain was increased by the addition of G3P. When G3P and glucose, glu cose-6-phosphate or fructose-6-phosphate were added, beta-galactosidas e activity was reduced showing that GlpP mediates catabolite repressio n of transcription from the glpD leader in the absence of any other B. subtilis protein. (C) 1998 Federation of European Microbiological Soc ieties. Published by Elsevier Science B.V.