CONSTITUTIVE CHOLINE TRANSPORT IN PSEUDOMONAS-AERUGINOSA

Pseudomonas aeruginosa has a choline uptake system which is expressed in bacteria grown in the presence of succinate and ammonium chloride a s the carbon and nitrogen source, respectively. This system obeys Mich aelis-Menten kinetics with an apparent K-m value of 53 mu M; its activ ity is not inhibited by high osmolarities in the medium but is partial ly inhibited by choline metabolites such as betaine and dimethylglycin e. (C) 1998 Federation of European Microbiological Societies. Publishe d by Elsevier Science B.V.