S. Morotomi et al., PHYSIOLOGICAL-PROPERTIES OF A NEUTRALO-SENSITIVE MUTANT DERIVED FROM FACULTATIVE ALKALIPHILIC BACILLUS SP. C-25, Bioscience, biotechnology, and biochemistry, 62(4), 1998, pp. 788-791
A neutralo-sensitive mutant (M-12) was isolated from the facultative a
lkaliphilic Bacillus sp. C-125. This mutant strain was able to grow to
the same extent as did the parent strain above pH 8, but did not grow
below pH 7.5. The same extent of oxygen uptake was shown by the cells
of the parent and mutant strains at DH 10.3. On the other hand, the o
xygen uptake rate was about one-fifth of that of the parent strain at
pH 7. NADH-dependent oxygen uptake by everted vesicles of the mutant w
as lower than that of the parent strain at pH 7-7.5, while the rate at
pH 8-9 was almost identical in both strains. The activity at pH 7 of
cytochrome c oxidase of right-side-out membrane vesicles of the mutant
strain was lower than that of the parent strain at pH 7, while both s
amples had almost the same enzymatic activity at pH 8.5. These results
suggest that poor respiratory activities of the mutant strain at pH 7
are the reason why this mutant strain was unable to grow at neutral p
H.