PURIFICATION AND PROPERTIES OF ACETYLACETOIN SYNTHASE FROM BACILLUS SP. YUF-4

Authors
UI S HOSAKA T MIZUTANI K MIMURA A
Citation
S. Ui et al., PURIFICATION AND PROPERTIES OF ACETYLACETOIN SYNTHASE FROM BACILLUS SP. YUF-4, Bioscience, biotechnology, and biochemistry, 62(4), 1998, pp. 795-797
Citations number
9
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
62
Issue
4
Year of publication
1998
Pages
795 - 797
Database
ISI
SICI code
0916-8451(1998)62:4<795:PAPOAS>2.0.ZU;2-C
Abstract
In Bacillus sp. YUF-4, acetylacetoin synthase was induced by acetoin, while glucose inhibited the induction. The enzyme was purified Ill-fol d by 6 purification steps, and a further purification followed, by HPL C using a TSK gel, Phenyl-5PW RP. The resulting enzyme gave a single b and with a molecular mass of 62 kDa by SDS-PAGE and 220 kDa by gel fil tration. Some enzymic characteristics were studied.