PURIFICATION OF POMACEA-CANALICULATA ALPHA-FUCOSIDASE ISOFORMS WITH DIFFERENT THERMOSTABILITIES

Authors
HIRATA K ASO Y YASUDA S ISHIGURO M
Citation
K. Hirata et al., PURIFICATION OF POMACEA-CANALICULATA ALPHA-FUCOSIDASE ISOFORMS WITH DIFFERENT THERMOSTABILITIES, Bioscience, biotechnology, and biochemistry, 62(4), 1998, pp. 807-810
Citations number
16
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
62
Issue
4
Year of publication
1998
Pages
807 - 810
Database
ISI
SICI code
0916-8451(1998)62:4<807:POPAIW>2.0.ZU;2-G
Abstract
Two isoforms, F1 (pI 4.7) and F2 (pI 4.9), of alpha-fucosidase were pu rified from the viscera of Pomacea canaliculata. The thermostability o f F1 was higher than that of F2. Both were 260-kDa proteins containing polypeptides (55 and 52 kDa) and sugar. There were small differences between F1 and F2 in the optimum conditions for the enzyme reaction an d pH stability.