OPTIMIZATION SEPARATION OF 5 PHOSPHOAMIDOTHIOATE ENANTIOMERS USING A SERIES OF SILICA AND CHIRAL COLUMN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A computer-assisted method is described for optimization of multi-comp onent, mobile phase selection for separation of five phosphoamidothioa te enantiomers with a series of silica and chiral columns in normal ph ase HPLC. The method is based on the triangular solvent selection conc ept using a statistical scanning method. The optimization of the separ ation over the experimental region is based on a special polynomial es timation from seven experimental runs, and resolution (R-s) is used as the selection criterion. Excellent agreement was obtained between pre dicted and experimental data.