COMBINED USE OF ANALYTICAL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ANDCELL MORPHOLOGY TRANSFORMATION ASSAY TO DETECT NEW-PROTEIN PHOSPHATASE INHIBITORS OF OKADAIC ACID TYPE
Yf. Pouchus et al., COMBINED USE OF ANALYTICAL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ANDCELL MORPHOLOGY TRANSFORMATION ASSAY TO DETECT NEW-PROTEIN PHOSPHATASE INHIBITORS OF OKADAIC ACID TYPE, Toxicon, 36(2), 1998, pp. 383-389
The search for new protein phosphatase inhibitors in shellfish contami
nated by toxin-producing dinoflagellates generally relies on prelimina
ry separation techniques followed by biological tests. To detect such
substances without purifying them initially, we developed an approach
based on a correlation of the results of two different analytical tech
niques applied to toxic extracts. high-performance liquid chromatograp
hy after derivation of the toxins and the cell morphology transformati
on assay on KB cells. Application of this protocol to stored frozen mu
ssels showed a decrease in okadaic acid concentration during storage,
with formation of degradation derivatives, some of which possessed not
able protein phosphatase inhibition activity. (C) 1998 Elsevier Scienc
e Ltd. All rights reserved.