EFFECT OF THE DIFFERENT PARTS OF THE CORN COB EMPLOYED AS A CARRIER ON LIGNINOLYTIC ACTIVITY IN SOLID-STATE CULTURES BY P-CHRYSOSPORIUM

Outside and inside corn cob were used to study ligninolytic enzymes pr oduced by Phanerachaete chrysosporium BKM-F-1767 (ATCC 24725) during s olid state fermentation conditions. In a previous work by employing a mixture of outside and inside corn cob, we achieved a maximum MnP acti vity of 96 U l(-1) but LiP activities were low. In the present work we determined which part of the corn cob is more suitable in order to ob tain high ligninolytic activities. We could find MnP activities about 300 U l(-1) by employing inside corn cob as a carrier and a maximum Li P activity of 24 U l(-1). In a subsequent experiment, using inside cor n cob as a carrier, we could considerably improve ligninolytic enzymes production, by supplementing the medium with Tween 80 (0.5% v/v). We obtained a maximum MnP activity of 384 U l(-1) and a maximum LiP activ ity of 155 U l(-1).