OPTIMIZATION STUDIES ON THE PRODUCTION OF CYCLOSPORINE-A BY SOLID-STATE FERMENTATION

Several parameters including (a) tray fermentation with and without pe rforation (b) thickness of solid substrate bed (c) type of inoculum (d ) size of inoculum (e) effect of relative humidity were studied for th e optimum production of Cyclosporin A by solid state fermentation usin g Tolypocladium sp. The results indicate that while perforations in th e trays had no significance on the yield of Cyc A, the other parameter s had an influence on the production of Cyc A. The results indicate th at under the optimized conditions, Cyc A can be produced in bulk quant ities economically.