QUANTITATION OF CYTIDINE 3',5'-CYCLIC-MONOPHOSPHATE IN AQUEOUS-SOLUTION BY UV ABSORPTION SPECTROPHOTOMETRY INDEPENDENT OF DIRECT DETERMINATION OF SOLUTION PH

uv absorbance spectrophotometry is the routine method of determining n ucleotide concentrations in solution. To obviate the need for determin ing solution pH a method is described whereby cyclic CMP concentration in aqueous solution is calculated from absorbances at four wavelength s: the rationale is of general applicability to nucleosides and nucleo tides.