ECTOPIC EXPRESSION OF LUNATIC FRINGE LEADS TO DOWN-REGULATION OF SERRATE-1 IN THE DEVELOPING CHICK NEURAL-TUBE - ANALYSIS USING IN OVO ELECTROPORATION TRANSFECTION TECHNIQUE

Citation
K. Sakamoto et al., ECTOPIC EXPRESSION OF LUNATIC FRINGE LEADS TO DOWN-REGULATION OF SERRATE-1 IN THE DEVELOPING CHICK NEURAL-TUBE - ANALYSIS USING IN OVO ELECTROPORATION TRANSFECTION TECHNIQUE, FEBS letters, 426(3), 1998, pp. 337-341
Citations number
26
Categorie Soggetti
Biology,"Cell Biology",Biophysics
Journal title
ISSN journal
00145793
Volume
426
Issue
3
Year of publication
1998
Pages
337 - 341
Database
ISI
SICI code
0014-5793(1998)426:3<337:EEOLFL>2.0.ZU;2-6
Abstract
Lunatic Fringe (l-Fng) is one of the vertebrate homologues of Drosophi la Fringe, which interacts with the Notch signal pathway and regulates activation of the Notch ligands, Delta and Serrate. To elucidate the roles of l-Fng in vertebrate neurogenesis, we transfected chick l-Fng (C-l-Fng) to chick neural tube using the in ovo electroporation techni que and examined the subsequent changes in expression of Notch-related genes. We observed downregulation of C-Serrate-1 by ectopic C-l-Fng e xpression which implied that C-l-Fng acts on the vertebrate Notch path way to regulate the expression of its ligand. (C) 1998 Federation of E uropean Biochemical Societies.