SUBCELLULAR PROPERTIES OF [CA2-DEFICIENT MOUSE VENTRICULAR CELLS(](I)TRANSIENTS IN PHOSPHOLAMBAN)

The regulatory protein phospholamban exerts a physiological inhibitory effect on the sarcoplasmic reticulum (SR) Ca2+ pump that is relieved with phosphorylation. We have studied the subcellular properties of in tracellular Ca2+ ([Ca2+](i)) transients in ventricular myocytes isolat ed from wild-type (WT) and phospholamban-deficient (PLB-KO) mice. In P LB-KO myocytes, steady-state twitch [Ca2+](i) transients revealed an a ccelerated relaxation and the occurrence of highly localized failures of Ca2+ release. The acceleration of SR Ca2+ uptake caused an increase in SR Ca2+ load with the frequent occurrence of spontaneous [Ca2+](i) waves and Ca2+ sparks. [Ca2+](i) waves in PLB-KO cells showed a marke d decrease in spatial width and more frequently appeared to abort. Loc al Ca2+ release events (Ca2+ sparks) were larger and more variable in amplitude and [Ca2+](i) declined faster in PLB-KO myocytes. Increased local buffering and reduction in the refractoriness of SR Ca2+ release caused by the increased SR pump rate led to an overall enhancement of local [Ca2+](i) gradients and inhomogeneities in the [Ca2+](i) distri bution during spontaneous Ca(2+)release, [Ca2+](i) waves, and excitati on-contraction coupling.