HPLC ANALYSIS OF THE PRODUCT DISTRIBUTION IN THE IODINE-CATALYZED METHYL GLYCOSIDATION OF PENTOSES AND 6-DEOXYHEXOSES

The product distribution of the iodine-catalyzed methyl glycosidation of four pentoses (D-ribose, D-arabinose, D-xylose, and D-lyxose) and t wo 6-deoxyhexoses (L-rhamnose, and D-fucose) was studied by HPLC using an APS column (dihydrogen sulphate form) with different acetonitrile- water mobile phases. In agreement with earlier results, a temperature dependent on-column isomerization was observed for all the investigate d aldoses, except for ribose. The first-eluted furanosides were follow ed by pyranosides, and the free sugars were eluted last with the highe st retention volumes.