QUANTITATIVE-ANALYSIS OF CARCINOEMBRYONIC ANTIGEN MESSENGER-RNA IN PERIPHERAL VENOUS-BLOOD AND PORTAL BLOOD OF PATIENTS WITH PANCREATIC DUCTAL ADENOCARCINOMA

One major therapeutic failure of pancreatic adenocarcinoma treatment i s metastasis to the liver, To screen patients with high risk for such hematogenous dissemination, we previously developed a very sensitive s ystem to detect carcinoembryonic antigen (CEA) in blood, For a more pr actical application, rye improved this system by making it quantitativ e and capable of analyzing both preoperative peripheral blood and intr aoperative portal blood for the presence of CEA mRNA, CEA mRNA was not detected in the peripheral venous blood of any of the three patients examined, but it was identified in the portal blood without fail, In a ddition, the quantities of CEA mRNA identified in the portal blood bef ore and after pancreatectomy were different. This study suggests that analysis of the portal blood seems to be important for the precise eva luation of hematogenous dissemination and of the pathophysiology of pa ncreatic ductal adenocarcinoma.