La. Prather et Rk. Jansen, PHYLOGENY OF COBAEA (POLEMONIACEAE) BASED ON SEQUENCE DATA FROM THE ITS REGION OF NUCLEAR RIBOSOMAL DNA, Systematic botany, 23(1), 1998, pp. 57-72
The phylogenetic relationships of 16 of the 18 species of the neotropi
cal genus Cobaea (Polemoniaceae) were investigated using sequences of
the ITS1 and ITS2 regions of nuclear ribosomal DNA. Cantua and Bonplan
dia were used as outgroups. Six most-parsimonious trees (length = 558
steps, CI excluding uninformative characters = 0.688, RI = 0.875) were
produced, and support for most branches, based on bootstrap and decay
analyses, was quite strong. Insertion/deletion events provide further
support for some clades. There are two unresolved nodes in the strict
consensus tree, and one of the unresolved nodes is among samples of o
ne species. The ITS phylogeny suggests that two of the three sections
of Cobaea are not monophyletic; the third is monotypic. The three char
acters used to distinguish the sections, corolla lobe shape, infloresc
ence position, and stamen length, were of questionable utility in sect
ional circumscription.